Composite

Part:BBa_M36394

Designed by: Dominique Dabija, Christopher Jackson, Debha Amatya   Group: Stanford BIOE44 - S11   (2011-12-06)

Spider Silk Actuator

Our part takes as input the PoPS signal generated by rhamnose, and the output is spider silk protein. This is a spider silk generating device that produces a hexamer of spider silk protein codon optimized for expression in E. coli. The DNA of the device is made up of Fos and Jun linked to the front and back of the spider silk hexamer "spilk 7.0." Fos and Jun are proteins with leucine zippers that form heterodimers in solution. The idea is that our E. coli cell will produce the hexamers of spider silk and have them aggregate together in very long chains bound together by Fos and Jun interactions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
    Illegal PstI site found at 510
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
    Illegal PstI site found at 510
    Illegal NotI site found at 302
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
    Illegal PstI site found at 510
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal PstI site found at 151
    Illegal PstI site found at 510
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 663


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Categories
Parameters
None